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[hal-03655943] CLIMTREE: Quantifying changes in flying insect diversity and soil fauna along a gradient of climate induced forest decline using DNA metabarcoding
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 30 Apr 2022
https://hal.inrae.fr/hal-03655943v1
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[hal-02609829] Dépérissement et diversité des insectes forestiers - une étude de cas dans les sapinières pyrénéennes
En raison de changements climatiques profonds, on constate une augmentation de fréquence et d'intensité des sécheresses dans certaines parties du globe (dont l'Europe) avec des conséquences déjà visibles sur les forêts. Dans ce contexte, le projet Climtree porte sur les Impacts écologiques et socio-économiques du dépérissement des arbres induit par les changements climatiques dans les forêts de montagne. Grâce à l'étude de 56 placettes dans 2 régions des Pyrénées, nous analysons ici la réponse de la diversité des assemblages entomologiques (i) à l'intensité du dépérissement local de la sapinière, (ii) au niveau de dépérissement des sapinières dans le paysage ? et (iii) aux coupes de récupération mises en oeuvre dans les peuplements dépérissants. Les premiers résultats montrent que le niveau de dépérissement local affecte la structure des sapinières, la composition générale des communautés d'insectes, l'abondance moyenne des xylo- et saproxylophages de résineux mais pas le nombre moyen d'espèces de coléoptères saproxyliques. Le niveau régional de dépérissement montre un effet significatif sur l'abondance des xylophages potentiels ravageurs de faiblesse, sur la richesse des xylo- et saproxylo-phages de résineux, l'abondance et la richesse moyenne des espèces rares et l'abondance moyenne des espèces cavicoles et fongicoles. La coupe de récupération ne provoque pas d'érosion générale de la diversité des assemblages de coléoptères saproxyliques et provoque de faibles changements de composition des communautés d'insectes.
ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 16 May 2020
https://hal.inrae.fr/hal-02609829v1
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[hal-03206694] Climate-induced forest dieback drives compositional change in insect communities that is concentrated amongst rare species
Marked decline in insect species richness, abundance and biomass have recently been quantified in Europe. We metabarcoded 224 Malaise-trap samples to investigate whether drought-induced forest dieback and subsequent salvage logging have an impact on flying insects (ca. 3000 insect species) in silver fir Pyrenean forests. We found no evidence that climate-induced forest dieback impacted species richness of flying insects but revealed compositional turnover patterns consistent with those seen during natural forest succession, given that the key covariates explaining compositional variation were canopy openness versus microhabitat diversity and deadwood amount at local and landscape scales, respectively. Importantly, most change was driven by rare species. In contrast, observed levels of salvage logging did not explain change in species richness or composition. Hence, although forest dieback appears to cause changes in species assemblages mimicking natural forest succession, it also increases the risk of catastrophic loss of rare species through homogenization of environmental conditions.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 23 Apr 2021
https://univ-tours.hal.science/hal-03206694v1
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[hal-02736320] DNA Metabarcoding to quantify the response of insect diversity to mountain forest die-offs in the French Pyrenees
Mountain forests suffer from an increase in frequency and severity of summer droughts and infestations of pathogens and insects. Those factors are causing high mortality of some keystone tree species (forest die-offs). Yet, how tree diebacks and associated changes in forest composition will affect local diversity and ecosystem functions remains unknown. Here, we aim at quantifying the impact of climate-induced forest decline on biodiversity by measuring changes in taxonomic structure of invertebrate communities along gradients of silver fir (Abies alba) dieback in the French Pyrenees. We use DNA metabarcoding to analyse 224 samples of Malaise traps placed on 56 silver fir dominated plots in the French Pyrenees from May to September 2017. Samples were sequenced using Illumina MiSeq and analysed using the DAMe twin-tagging pipeline approach. We conducted taxonomic assignment against reference DNA barcode libraries to streamli ne identification and recover biological information for ecological analysis. We discuss the results of our metabarcoding analysis and the utility of our approach to conduct biomonitoring across a large geographical scale.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 02 Jun 2020
https://hal.inrae.fr/hal-02736320v1
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[hal-03655983] Non-Destructive DNA metabarcoding of insects for forest biomonitoring
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 30 Apr 2022
https://hal.inrae.fr/hal-03655983v1
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[hal-02787977] WP1 Impacts on biodiversity - French site
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 05 Jun 2020
https://hal.inrae.fr/hal-02787977v1
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[hal-03079078] Contrasting responses of habitat conditions and insect biodiversity to pest- or climate-induced dieback in coniferous mountain forests
Natural disturbances are major drivers of forest dynamics. However, in the current context of anthropogenic global warming, shifts in disturbance regimes are expected. Natural disturbances usually leave biological or structural legacies which are important for early-successional species. Nevertheless, these legacies are usually eliminated by forest managers through salvage logging. Here, we investigated the consequences of forest dieback and the following salvage logging on both forest habitat conditions and saproxylic beetle communities. We conducted our study in two types of conifer-dominated highland forests: Pyrenean silver fir (Abies alba) which has suffered drought-induced dieback and Bavarian Norway spruce (Picea abies) which has suffered bark beetle-induced (Ips typographus) dieback. In both of the forest contexts, dieback provided a biological legacy through an increase in deadwood resources; however, this increase was much greater in the spruce forests. Nonetheless, despite this increase in resources, neither type of forest gained in total abundance or species richness after disturbance, compared to healthy stands. Nevertheless, the species composition of saproxylic beetle composition was significantly affected by dieback in spruce stands, but not in the silver fir forests. In the spruce plots, saproxylic beetles responded positively to the large increase in deadwood in the declining stands, including a very strong positive response from red listed species. Saproxylic beetle assemblages in spruce forests were mainly drove by canopy openness and deadwood amount. In the silver fir plots, we did not observed responses from the saproxylic beetle communities to deadwood amount increase. This lack of response may be explained by the relatively low amount of deadwood generated by the drought-induced dieback. Concerning salvage logging, it caused stronger contrasts in spruce forests than in silver fir forests, where it generally had no significant impact. For example, in spruce forests, salvage logging reduced the density of large snags by 91% and large logs by 87% compared with unharvested declining plots. Most of the significant environmental effects on biodiversity associated with dieback were no longer significant after accounting for the salvaged plots in our study data. Then, forest dieback and salvage logging induced much sharper and stronger effects on environmental and community metrics in the spruce than in the silver fir forests. The contrast between Bavaria and the French Pyrenees seems partly related to dieback severity. Finally, we invite forest managers to conserve biological and structural legacies through patches of deadwood-rich areas.
ano.nymous@ccsd.cnrs.fr.invalid (Jérémy Cours) 07 Jun 2024
https://hal.inrae.fr/hal-03079078v1
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[hal-03881017] Multi-omics and multi-tissues data to improve the understanding of heat stress adaptation mechanisms
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Guilhem Huau) 01 Dec 2022
https://hal.science/hal-03881017v1
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[hal-03186538] The ecological impact of pest-induced tree dieback on insect biodiversity in Yunnan pine plantations, China
China has recently announced a reform of forestry policy, with a major goal being to transform from plantation to heterogeneous forests, which have higher resistance to pests and disease and house more biodiversity. One driver of reform is increased intensity and frequency of pest-induced tree-dieback events. To inform management, we ask what effects these events have on insect biodiversity in Pinus yunnanensis monocultures in Yunnan province, the province with one of the highest proportions of forest cover in China. We sampled aerial arthropods (mostly insect) biodiversity along gradients of Pinus yunnanensis dieback severity using Malaise traps and used metabarcoding to characterise the insect community. We used MS-GDM ('multi-site generalized dissimilarity modelling of zeta diversity'), zeta-decline analysis, and iNEXT ('Interpolation and extrapolation for species diversity') to assess community change as functions of forest-structure covariates. Metabarcoding of Malaisetrapped insects reveals that bark-beetle induced forest dieback does not result in detectable differences in species diversity but does result in compositional change, with the biggest turnover occurring between 0% and infested-0%-open-canopy forests and 20%-infested-20%-open-canopy forests. Zeta-decline analysis found that the insect community in low-infestation forests is characterized by a stochastic assembly, while in highinfestation forests, the community structure is consistent with niche assembly. Our results thus suggest that bark-beetle dieback mimics natural forest-gap dynamics, consistent with the interpretation of bark beetles as a keystone species in European conifer forests, where it has been proposed that forest heterogeneity can be created efficiently by allowing natural disturbances, including bark-beetle outbreaks, to proceed naturally, without being mitigated by deadwood removal and dense replanting. In Yunnan's situation, and given predicted increases in bark-beetle dieback severity and frequency, this strategy should probably be supplemented with anthropogenic treatments, such as deadwood enhancement and planting of multiple tree species, to accelerate the succession of plantations into heterogeneous forests.
ano.nymous@ccsd.cnrs.fr.invalid (Wang Cai) 31 Mar 2021
https://hal.inrae.fr/hal-03186538v1
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[hal-04171539] Worldwide tests of generic attractants, a promising tool for early detection of non-native cerambycid species
A large proportion of the insects which have invaded new regions and countries are emerging species, being found for the first time outside their native range. Being able to detect such species upon arrival at ports of entry before they establish in non-native countries is an urgent challenge. The deployment of traps baited with broad-spectrum semiochemical lures at ports-of-entry and other high-risk sites could be one such early detection tool. Rapid progress in the identification of semiochemicals for cerambycid beetles during the last 15 years has revealed that aggregation-sex pheromones and sex pheromones are often conserved at global levels for genera, tribes or subfamilies of the Cerambycidae. This possibly allows the development of generic attractants which attract multiple species simultaneously, especially when such pheromones are combined into blends. Here, we present the results of a worldwide field trial programme conducted during 2018–2021, using traps baited with a standardised 8-pheromone blend, usually complemented with plant volatiles. A total of 1308 traps were deployed at 302 sites covering simultaneously or sequentially 13 European countries, 10 Chinese provinces and some regions of the USA, Canada, Australia, Russia (Siberia) and the Caribbean (Martinique). We intended to test the following hypotheses: 1) if a species is regularly trapped in significant numbers by the blend on a continent, it increases the probability that it can be detected when it arrives in other countries/continents and 2) if the blend exerts an effective, generic attraction to multiple species, it is likely that previously unknown and unexpected species can be captured due to the high degree of conservation of pheromone structures within related taxa. A total of 78,321 longhorned beetles were trapped, representing 376 species from eight subfamilies, with 84 species captured in numbers greater than 50 individuals. Captures comprised 60 tribes, with 10 tribes including more than nine species trapped on different continents. Some invasive species were captured in both the native and invaded continents. This demonstrates the potential of multipheromone lures as effective tools for the detection of ‘unexpected’ cerambycid invaders, accidentally translocated outside their native ranges. Adding new pheromones with analogous well-conserved motifs is discussed, as well as the limitations of using such blends, especially for some cerambycid taxa which may be more attracted by the trap colour or other characteristics rather than to the chemical blend.
ano.nymous@ccsd.cnrs.fr.invalid (Alain Roques) 27 Jul 2023
https://hal.inrae.fr/hal-04171539v1
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[hal-04186469] Communautés d’insectes et changement global : enjeux méthodologiques et écologiques
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ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 23 Aug 2023
https://hal.inrae.fr/hal-04186469v1
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[hal-02788069] Quantifying the impact of tree-diebacksand salvage logging on mountain forest biodiversity using metabarcoding
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 05 Jun 2020
https://hal.inrae.fr/hal-02788069v1
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[hal-02734096] Does forest biodiversity respond to pulses of saproxylic microhabitats induced by tree dieback: a case study in mountain French silver fir forests
Forest diebacks are likely to increase in response to climate change, with increased frequency and intensity of droughts. In line with climate change scenarios, ecoclimatic modelling predicts a decrease in the range of silver fir, a drought-sensitive species, in its southern limit in the French Pyrenees. Diebacks are expected to induce a pulse of resources potentially favorable to certain forest species, e.g. tree-related microhabitats (TreM) for saproxylic species. The impact of forest dieback on biodiversity has nonetheless been poorly studied. As part of the international Climtree project, we set up a balanced sampling design of 56 plots crossing the intensity of local silver fir dieback and the salvage logging of weakened or dead trees. Detailed stand structure metrics and insect communities sampled by Malaise traps (insect MOTUs) or flight-interception traps (saproxylic beetles) have been measured. The structure of fir stands was affected by the level of decline, and to a lesser extent by salvage logging. We indeed observed a slight increase in CWD and in some TreM-bearing trees (crown deadwood, annual polypores , trunk rot holes) with dieback intensity, and a slight decrease in some TreM-bearing trees (crown deadwood, annual polypores) in salvaged compared with unharvested plots. However, these stand changes did not strongly affect local insect assemblages. Guilds of TreM-associated insects did not increase in abundance or richness with dieback-induced increase in resources. In conclusion, forest changes through dieback result in habitat and resource changes with still hard-to-predict impacts at the stand scale on a major reservoir of biodiversity.
ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 02 Jun 2020
https://hal.inrae.fr/hal-02734096v1
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[hal-02609830] Dépérissement et diversité des insectes forestiers - une étude de cas dans les sapinières pyrénéennes
En raison des changements climatiques profonds actuels, on constate une augmentation de fréquence et d'intensité des sécheresses en Europe avec des conséquences déjà visibles sur les forêts. Dans ce contexte, le projet Climtree porte sur les Impacts écologiques et socio-économiques du dépérissement des arbres induit par les changements climatiques dans les forêts de montagne. Grâce à l'étude de 56 placettes dans 2 régions des Pyrénées, nous analysons ici la réponse de la diversité des assemblages entomologiques (i) à l'intensité du dépérissement local de la sapinière, (ii) au niveau de dépérissement des sapinières dans le paysage ? et (iii) aux coupes de récupération mises en oeuvre dans les peuplements dépérissants. Les premiers résultats montrent que le niveau de dépérissement local affecte la structure des sapinières, et la composition générale des communautés d'insectes mais pas le nombre moyen d'OTUs d'insectes. Le niveau régional de dépérissement montre un effet significatif sur plusieurs métriques décrivant l'assemblage de coléoptères saproxyliques : l'abondance des xylophages potentiels ravageurs de faiblesse, la richesse des xylo- et saproxylo-phages de résineux, l'abondance et la richesse moyenne des espèces rares et l'abondance moyenne des espèces cavicoles et fongicoles. La coupe de récupération ne provoque pas d'érosion générale de la diversité des assemblages de coléoptères saproxyliques et provoque seulement de faibles changements de composition des communautés d'insectes.
ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 16 May 2020
https://hal.inrae.fr/hal-02609830v1
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[hal-02609828] Do Tree-related Microhabitats and associated biodiversity respond to forest dieback? A case study in French mountain Silver Fir forests
Forest diebacks are likely to increase in response to climate change, with increased frequency and intensity of droughts. In line with climate change scenarios, ecoclimatic modelling predicts a decrease in the range of silver fir, a drought-sensitive species, in its southern limit in the French Pyrenees. Diebacks are expected to induce a pulse of resources potentially favorable to certain forest species, e.g. tree-related microhabitats (TreM) for saproxylic species. The impact of forest dieback on biodiversity has nonetheless been poorly studied. As part of the international Climtree project, we set up a balanced sampling design of 56 plots crossing the intensity of local silver fir dieback and the salvage logging of weakened or dead trees. Detailed stand structure metrics and insect communities sampled by Malaise traps (insect MOTUs) or flight-interception traps (saproxylic beetles) have been measured. The structure of fir stands was affected by the level of decline, and to a lesser extent by salvage logging. We indeed observed a slight increase in CWD and in some TreM-bearing trees (crown deadwood, annual polypores , trunk rot holes) with dieback intensity, and a slight decrease in some TreM-bearing trees (crown deadwood, annual polypores) in salvaged compared with unharvested plots. However, these stand changes did not strongly affect local insect assemblages. Guilds of TreM-associated insects did not increase in abundance or richness with dieback-induced increase in resources. In conclusion, forest changes through dieback result in habitat and resource changes with still hard-to-predict impacts at the stand scale on a major reservoir of biodiversity.
ano.nymous@ccsd.cnrs.fr.invalid (Christophe Bouget) 16 May 2020
https://hal.inrae.fr/hal-02609828v1
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[hal-03655945] Insect response to climate-induced tree die-offs in the French Pyrenees
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 30 Apr 2022
https://hal.inrae.fr/hal-03655945v1
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[hal-03475845] Drought-induced forest dieback increases taxonomic and functional diversity but not phylogenetic diversity of saproxylic beetles at both local and landscape scales
Context: Forest ecosystems worldwide are facing increasing drought-induced dieback, causing mortality patches across the landscape at multiple scales. This increases the supply of biological legacies and differentially affects forest insect communities. Objectives: We analysed the relative effects of local- and landscape-level dieback on local saproxylic beetle assemblages. We assessed how classic concepts in spatial ecology (e.g. habitat-amount and habitat-patch hypotheses) are involved in relationships between multi-scale spatial patterns of available resources and local communities. Methods: We sampled saproxylic beetle assemblages in commercial fir forests in the French highlands. Through automatic aerial mapping, we used dead tree crowns to assess dieback levels at several nested spatial scales. We analysed beetle taxonomic, phylogenetic and functional diversity related to differing levels of multi-scale dieback. Results: In line with the habitat-amount hypothesis, taxonomic and functional diversity, but not phylogenetic diversity, of beetle assemblages significantly benefitted from forest dieback, at both local and landscape scales. Very few single or interaction effects were detected in the multiplicative models combining local and landscape variables, though a significant positive effect of landscape-scale dieback on the abundance of cavity- and fungus-dwelling species was consistent with a spill-over effect. Increased landscape-scale dieback also caused a functional specialisation of beetle assemblages, favouring those related to large-diameter, well-decayed deadwood. Conclusions: Increasing tree mortality under benign neglect provides conservation benefits by heterogenising the forest landscape and enhancing deadwood habitats. Legacy retention practices could take advantage of unharvested, declining forest stands to promote species richness and functional diversity within conventionally managed forest landscapes.
ano.nymous@ccsd.cnrs.fr.invalid (Jérémy Cours) 11 Dec 2021
https://hal.inrae.fr/hal-03475845v1
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[hal-03655928] DNA metabarcoding to quantify the ecological impact of forest decline on flying insect diversity in the Pyrenees
Background: Forests suffer from an increase in frequency and severity of summer droughts and infestations of pathogens and insects.Those factors cause high mortality of some keystone tree species (forest die-offs). Yet, how tree mortality and associated changes in forestcomposition will affect local diversity and ecosystem functions remains unknown. Here, we aim at quantifying the impact of climate-induced forest decline on biodiversity by measuring changes in the taxonomic structure of invertebrate communities along silver fir(Abies alba) dieback and salvage logging gradients in the French Pyrenees. We examine patterns of variation in species diversity of flyinginsect assemblages collected by Malaise traps deployed in 57 silver fir-dominated experimental plots (one Malaise trap per plot) in thecentral and eastern Pyrenees. Sampling was carried out each month for over 4 months (May–August 2017). Samples were sequenced usingIllumina MiSeq and analyzed using the DAMe twin-tagging pipeline approach. Results: We obtained 224 bulk samples filled with a solu-tion of monopropylene glycol plus ethanol. Despite high levels of DNA degradation detected in our samples, we found no major impact onspecies detection, with more than 3500 operational taxonomic units (OTUs) in 18 different insect orders recovered. We found large speciestemporal turnover (Jaccard Index: May–August = 0.35), as well as changes in community composition but no significant loss of speciesdiversity along the forest decline gradient. Significance: There is an urgent need to obtain detailed baseline data on species assemblages to quantify the impacts of climate change. Our study assessed biodiversity patterns on a scale and with a resolution that was previouslyimpossible and provides data essential for evaluating future biotic change. Our workflow coupling metabarcoding and Malaise trapping is simple to use and provides an affordable, reliable, and verifiable way of monitoring forest biodiversity at a large geographical scale.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 30 Apr 2022
https://hal.inrae.fr/hal-03655928v1
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[hal-02613360] Understanding the impacts of widespread forest die-offs across France, Germany, and China
Metabarcoding increases the taxonomic resolution and geographic scale at which researchers can assess the impacts of climate change on insect communities in forests
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 20 May 2020
https://hal.inrae.fr/hal-02613360v1
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[hal-02787654] DNA Metabarcoding to quantify the ecological impact of climate-induced forest diebacks in the Pyrenees
[...]
ano.nymous@ccsd.cnrs.fr.invalid (Paul Schmidt Yanez) 05 Jun 2020
https://hal.inrae.fr/hal-02787654v1
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[hal-03533022] Plasticity of feeding behaviour traits in response to production environment (temperate vs. tropical) in group-housed growing pigs
Heat stress affects pig metabolism, health and welfare, resulting in reduced growth and important economic losses. The present experiment aimed to evaluate the effects of two climatic environments [temperate (TEMP) vs. tropical humid (TROP)] on feeding behaviour in growing pigs. The feeding behaviour traits were measured with automated feeders and included: daily feed intake, daily eating time, feeding rate, daily number of meals, feed intake per meal, and feeding time per meal. Pigs came from a backcross population between Large White (LW, heat sensitive) and Creole (CR, heat tolerant) pigs. The same 10 F1 LW × CR boars (sire families [SF]) were mated with related LW sows in each environment. Feeding behaviour was recorded for a total of 1,296 pigs (n = 634 pigs for TEMP and n = 662 pigs for TROP) between 11 and 23 weeks of age. Growth performance and thermoregulatory responses (rectal and skin temperatures) were also measured. Results show that TROP conditions affect feeding behaviour traits: animals had more meals per day but these meals were smaller both in duration and in size, resulting in lower daily feed intake and less time eating per day. Significant SF by environment (GxE) interactions were found for all feeding behaviour traits. When SF were distributed into robust and sensitive groups (previously defined according to performance and thermoregulatory traits), results showed group by environment interactions for all feeding traits, except meal frequency. Moreover, a significant difference in feeding rate between robust and sensitive group was detected in TEMP, suggesting that feeding rate may be a good candidate to evaluate heat tolerance.
ano.nymous@ccsd.cnrs.fr.invalid (Nausicaa Poullet) 25 Jul 2023
https://hal.inrae.fr/hal-03533022v1
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[hal-04169150] Non-destructive DNA metabarcoding of arthropods using collection medium from passive traps
Broad-scale monitoring of arthropods is often carried out with passive traps ( e.g . Malaise traps) that can collect thousands of specimens per sample. The identification of individual specimens requires time and taxonomic expertise, limiting the geographical and temporal scale of research and monitoring studies. DNA metabarcoding of bulk-sample homogenates is faster and has been found to be efficient and reliable, but is destructive and prevents a posteriori validation of species occurrences and/or relative abundances. Non-destructive DNA metabarcoding from the collection medium has been applied in a limited number of studies, but further tests of efficiency are required in a broader range of circumstances to assess the consistency of the method. Methods We quantified the detection rate of arthropod species when applying non-destructive DNA metabarcoding with a short (127-bp) fragment of mitochondrial COI on two types of passive traps and collection media: 1) water with monopropylene glycol (H 2 O–MPG) used in window-flight traps (WFT, 53 in total); 2) ethanol with monopropylene glycol (EtOH–MPG) used in Malaise traps (MT, 27 in total). We then compared our results with those obtained for the same samples using morphological identification (for WFTs) or destructive metabarcoding of bulk homogenate (for MTs). This comparison was applied as part of a larger study of arthropod species richness in silver fir ( Abies alba ) stands across a range of climate-induced tree dieback levels and forest management strategies. Results Of the 53 H 2 O-MPG samples from WFTs, 16 produced no metabarcoding results, while the remaining 37 samples yielded 77 arthropod MOTUs in total. None of those MOTUs were shared species with the 389 morphological taxa (343 of which were Coleoptera) obtained from the same traps. Metabarcoding of 26 EtOH–MPG samples from MTs detected more arthropod MOTUs (233) and insect orders (11) than destructive metabarcoding of homogenate (146 MOTUs, 8 orders). Arachnida and Collembola were more diverse in EtOH-MPG samples, but Hymenoptera, Coleoptera and Lepidoptera were less represented than in homogenate. Overall, MOTU richness per trap similar for EtOH–MPG (21.81 MOTUs) than for homogenate (32.4 MOTUs). Arthropod communities from EtOH–MPG and homogenate metabarcoding were relatively distinct, with 162 MOTUs (53%) unique to the collection medium and only 71 MOTUs (23%) present in both treatments. Finally, collection medium did not reveal any significant changes in arthropod richness along a disturbance gradient in silver fir forests. We conclude that DNA metabarcoding of collection medium can be used to complement homogenate metabarcoding in inventories to favour the detection of soft-bodied arthropods like spiders.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 24 Jul 2023
https://hal.inrae.fr/hal-04169150v1
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[hal-03700534] Drought-induced forest dieback increases taxonomic, functional, and phylogenetic diversity of saproxylic beetles at both local and landscape scales
Context: Many forest ecosystems around the world are facing increasing drought-induced dieback, causing mortality patches across the landscape at multiple scales. This increases the supply of biological legacies and differentially affects forest insect communities. Objectives; We analysed the relative effects of local-and landscape-level dieback on local saproxylic beetle assemblages. We assessed how classical concepts in spatial ecology (e.g., habitat-amount and habitat-patch hypotheses) are involved in relationships between multi-scale spatial patterns of available resources and local communities. Methods: We sampled saproxylic beetle assemblages in commercial fir forests in the French highlands. Through automatic aerial mapping, we used percentage of dead tree crown pixels to assess dieback levels at several nested spatial scales. We analysed beetle taxonomic, phylogenetic and functional diversity related to differing levels of multi-scale dieback. Results: We found that taxonomic, functional, and phylogenetic diversity of saproxylic beetle assemblages significantly benefitted from forest dieback, at both local and landscape scales. We detected significant effects in the multiplicative models combining local and landscape variables only for phylogenetic diversity. Increased landscape-scale dieback also caused a functional specialisation of beetle assemblages, favouring those related to large and welldecayed deadwood. Conclusions: Increasing tree mortality under benign neglect provides conservation benefits by heterogenising the forest landscape and enhancing deadwood habitats. Legacy retention practices could take advantage of unharvested, declining forest stands to promote species richness and functional diversity within conventionally managed forest landscapes.
ano.nymous@ccsd.cnrs.fr.invalid (Jérémy Cours) 07 Jun 2024
https://hal.inrae.fr/hal-03700534v1
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[hal-04385991] Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps
Background Broad-scale monitoring of arthropods is often carried out with passive traps ( e.g., Malaise traps) that can collect thousands of specimens per sample. The identification of individual specimens requires time and taxonomic expertise, limiting the geographical and temporal scale of research and monitoring studies. DNA metabarcoding of bulk-sample homogenates has been found to be faster, efficient and reliable, but the destruction of samples prevents a posteriori validation of species occurrences and relative abundances. Non-destructive metabarcoding of DNA extracted from collection medium has been applied in a limited number of studies, but further tests of efficiency are required with different trap types and collection media to assess the consistency of the method. Methods We quantified the detection rate of arthropod species when applying non-destructive DNA metabarcoding with a short (127-bp) fragment of mitochondrial COI on two combinations of passive traps and collection media: (1) water with monopropylene glycol (H 2 O–MPG) used in window-flight traps (WFT, 53 in total); (2) ethanol with monopropylene glycol (EtOH–MPG) used in Malaise traps (MT, 27 in total). We then compared our results with those obtained for the same samples using morphological identification (for WFTs) or destructive metabarcoding of bulk homogenate (for MTs). This comparison was applied as part of a larger study of arthropod species richness in silver fir ( Abies alba Mill., 1759) stands across a range of climate-induced tree dieback levels and forest management strategies. Results Of the 53 H 2 O-MPG samples from WFTs, 16 produced no metabarcoding results, while the remaining 37 samples yielded 77 arthropod MOTUs in total, of which none matched any of the 343 beetle species morphologically identified from the same traps. Metabarcoding of 26 EtOH–MPG samples from MTs detected more arthropod MOTUs (233) than destructive metabarcoding of homogenate (146 MOTUs, 8 orders), of which 71 were shared MOTUs, though MOTU richness per trap was similar between treatments. While we acknowledge the failure of metabarcoding from WFT-derived collection medium (H 2 O–MPG), the treatment of EtOH-based Malaise trapping medium remains promising. We conclude however that DNA metabarcoding from collection medium still requires further methodological developments and cannot replace homogenate metabarcoding as an approach for arthropod monitoring. It can be used nonetheless as a complementary treatment when enhancing the detection of soft-bodied arthropods like spiders and Diptera.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 12 Jan 2024
https://hal.inrae.fr/hal-04385991v1
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[hal-03555250] Climate-induced forest dieback drives compositional changes in insect communities that are more pronounced for rare species
Species richness, abundance and biomass of insects have recently undergone marked declines in Europe. We metabarcoded 211 Malaise-trap samples to investigate whether drought-induced forest dieback and subsequent salvage logging had an impact on ca. 3000 species of flying insects in silver fir Pyrenean forests. While forest dieback had no measurable impact on species richness, there were significant changes in community composition that were consistent with those observed during natural forest succession. Importantly, most observed changes were driven by rare species. Variation was explained primarily by canopy openness at the local scale, and the tree-related microhabitat diversity and deadwood amount at landscape scales. The levels of salvage logging in our study did not explain compositional changes. We conclude that forest dieback drives changes in species assemblages that mimic natural forest succession, and markedly increases the risk of catastrophic loss of rare species through homogenization of environmental conditions.
ano.nymous@ccsd.cnrs.fr.invalid (Lucas Sire) 03 Feb 2022
https://hal.inrae.fr/hal-03555250v1
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[hal-04449380] Multi-tissue metabolic and transcriptomic responses to a short-term heat stress in swine
Background Heat stress (HS) is an increasing threat for pig production with a wide range of impacts. When submitted to high temperatures, pigs will use a variety of strategies to alleviate the effect of HS. While systemic adaptations are well known, tissue-specific changes remain poorly understood. In this study, thirty-two pigs were submitted to a 5-day HS at 32 °C. Results Transcriptomic and metabolomic analyses were performed on several tissues. The results revealed differentially expressed genes and metabolites in different tissues. Specifically, 481, 1774, 71, 1572, 17, 164, and 169 genes were differentially expressed in muscle, adipose tissue, liver, blood, thyroid, pituitary, and adrenal glands, respectively. Regulatory glands (pituitary, thyroid, and adrenal) had a lower number of regulated genes, perhaps indicating an earlier sensitivity to HS. In addition, 7, 8, 2, and 8 metabolites were differentially produced in muscle, liver, plasma, and urine, respectively. The study also focused on the oxidative stress pathway in muscle and liver by performing a correlation analysis between genes and metabolites. Conclusions This study has identified various adaptation mechanisms in swine that enable them to cope with heat stress (HS). These mechanisms include a global decrease in energetic metabolism, as well as changes in metabolic precursors that are linked with protein and lipid catabolism and anabolism. Notably, the adaptation mechanisms differ significantly between regulatory (pituitary, thyroid and adrenal glands) and effector tissues (muscle, adipose tissue, liver and blood). Our findings provide new insights into the comprehension of HS adaptation mechanisms in swine.
ano.nymous@ccsd.cnrs.fr.invalid (Guilhem Huau) 12 Feb 2024
https://hal.inrae.fr/hal-04449380v1
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[anses-04353534] Avis de l'Anses relatif à « la catégorisation de Xylotrechus chinensis »
Dans le cadre de la surveillance des organismes réglementés ou émergents (SORE), un dispositif de piégeage a été déployé par la DGAL en 2021 avec l’appui de l’INRAE et de l’ONF. L’objectif de ce dispositif est d’effectuer une surveillance passive dans les sites d’entrée potentiels (ports, aéroports, marchés d’intérêt national (MIN) des organismes réglementés ou émergents (SORE). Ce piégeage est qualifié de « large spectre » car il peut concerner plusieurs filières de production suivies dans le cadre de la SORE (forêts, jardins et espaces verts et infrastructures (JEVI), arboriculture fruitière) en ciblant cependant majoritairement les insectes coléoptères des ligneux. Les principes de la surveillance mise en œuvre s’appuient sur les résultats précédemment acquis dans le cadre du projet PORTRAP, utilisant des pièges génériques multi-composés pour la détection précoce d’insectes exotiques xylophages dans les sites potentiels d’entrée sur le territoire national. Les pièges ont été disposés sur 13 sites (7 ports maritimes, 1 port fluvial, 4 aéroports et 1 marché national) dispersés sur le territoire (France continentale). Au total 9279 individus appartenant à 110 espèces différentes ont été capturés. Aucune espèce d’insecte de quarantaine prioritaire n’a été piégée. En revanche, la présence d’individus appartenant à 8 espèces de coléoptères exotiques, a priori non répandues sur notre territoire, des familles Cerambycidae et Curculionidae (sous-familles des Scolytinae et Platypodinae) a été relevée. Les 8 espèces d’insectes exotiques sont les suivantes : Cerambycidae : Cordylomera spinicornis (Fabricius, 1775), Trichoferus campestris (Faldermann, 1835), Xylotrechus chinensis (Chevrolat, 1852), Xylotrechus stebbingi (Gahan, 1906) ; Curculionidae : Amasa sp. near truncata, Euplatypus hintzii (Schaufuss, 1897), Euplatypus parallelus (Fabricius, 1801), Xyleborus affinis Eichhoff, 1868. Ainsi, il est demandé la réalisation d’une catégorisation des 8 espèces d’insectes exotiques en utilisant les critères de risque listés ci-dessous, afin de déterminer leur nuisibilité et de prioriser sur cette base la réalisation d’analyses de risque portant sur ces insectes.
ano.nymous@ccsd.cnrs.fr.invalid (Jean-Claude Grégoire) 19 Dec 2023
https://anses.hal.science/anses-04353534v1
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[hal-01583805] Conséquences des travaux d'entretien du lit de la Loire sur plusieurs composantes de la biodiversité au sein de la mosaïque des îles de Mareau-aux-Prés (Loiret).
Ce projet de recherche a étudié les conséquences des travaux d’entretien du lit de la Loire sur plusieurs composantes de la biodiversité (faune et flore) au sein de la mosaïque d’ilots de 13 hectares de Mareau-aux-Prés (inclus dans la Réserve Naturelle Nationale de St-Mesmin, Loiret). Suite aux travaux (dévégétalisation et reprofilage), un nouvel assemblage de communautés s’est mis progressivement en place. Ce projet est centré sur cette nouvelle dynamique de recolonisation: la biodiversité intra et interspécifique a été étudiée pendant 3 ans avant et après travaux (via l’acquisition de données in situ et d’analyses génétiques et spatialisées) autour de quatre compartiments biologiques : (1) végétation herbacée, arbustive et arborescente, autochtone et exotique (2) entomofaune coléoptère Carabidae (3) avifaune nicheuse des grèves (4) castor d’Europe. Nous avons pu observer et mesurer une succession quasi primaire alimentée par le flux de populations provenant des autres ilots, îles et berges.
ano.nymous@ccsd.cnrs.fr.invalid (Marc Villar) 07 Sep 2017
https://hal.science/hal-01583805v1
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[hal-02740183] Conséquences des travaux d'entretien du lit de la Loire sur plusieurs composantes de la biodiversité au sein de la mosaïque des îles de Mareau-aux-Prés (Loiret).
Ce projet de recherche a étudié les conséquences des travaux d’entretien du lit de la Loire sur plusieurs composantes de la biodiversité (faune et flore) au sein de la mosaïque d’ilots de 13 hectares de Mareau-aux-Prés (inclus dans la Réserve Naturelle Nationale de St-Mesmin, Loiret). Suite aux travaux (dévégétalisation et reprofilage), un nouvel assemblage de communautés s’est mis progressivement en place. Ce projet est centré sur cette nouvelle dynamique de recolonisation: la biodiversité intra et interspécifique a été étudiée pendant 3 ans avant et après travaux (via l’acquisition de données in situ et d’analyses génétiques et spatialisées) autour de quatre compartiments biologiques : (1) végétation herbacée, arbustive et arborescente, autochtone et exotique (2) entomofaune coléoptère Carabidae (3) avifaune nicheuse des grèves (4) castor d’Europe. Nous avons pu observer et mesurer une succession quasi primaire alimentée par le flux de populations provenant des autres ilots, îles et berges.
ano.nymous@ccsd.cnrs.fr.invalid (Marc M. Villar) 03 Jun 2020
https://hal.inrae.fr/hal-02740183v1
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[hal-01423445] Conséquences des travaux d'entretien du lit de la Loire sur plusieurs composantes de la biodiversité au sein de la mosaïque des îles de Mareau-aux-Prés (Loiret)
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ano.nymous@ccsd.cnrs.fr.invalid (Marc Villar) 29 Dec 2016
https://hal.science/hal-01423445v1
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[hal-02626409] Taxonomic revision of Neotropical Phyllocnistis Zeller, 1848 (Lepidoptera: Gracillariidae), with descriptions of seven new species and host plant associations
Until now, 20 species of leaf-mining micromoths of the genus Phyllocnistis Zeller (Lepidoptera, Gracillariidae) have been known to occur in the Neotropical region. Here we revise the previously known species and describe seven new species: four from French Guiana, P. kawakitai Brito & Lopez-Vaamonde, sp. nov., P. norak Brito & Lopez-Vaamonde, sp. nov., P. ohshimai Brito & Lopez-Vaamonde, sp. nov., P. petronellii Brito & Lopez-Vaamonde, sp. nov.; and, three from Brazil, P. helios Brito & Moreira, sp. nov., P. jupiter Brito & Moreira, sp. nov. and P. xylopiella Brito & Becker, sp. nov. Lectotypes are designated for P. aurilinea Zeller, 1877; P. citrella Stainton, 1856; P. rotans and P. sexangula Meyrick, 1915. Detailed descriptions of the pattern of forewing fasciae are provided for all species. Host plant associations, photographs of adults and illustrations of genitalia, when available, are provided for the described species of Neotropical Phyllocnistis. In addition, DNA barcodes were used for the delimitation of some species.
ano.nymous@ccsd.cnrs.fr.invalid (Rosangela Brito) 26 May 2020
https://hal.inrae.fr/hal-02626409v1
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[hal-02637388] The EASIN Editorial Board: quality assurance, exchange and sharing of alien species information in Europe
The European Alien Species Information Network (EASIN) aims to facilitate the exploration of alien species information in Europe, and is recognized as the information system supporting European Union Member States in the implementation of the recently published Invasive Alien Species Regulation. In this paper, we present the role and activities of the EASIN Editorial Board (EB), which is responsible for the quality assurance, safeguarding and constant improvement of EASIN. The EB is supported by a web platform that facilitates online discussions about alien species. This platform creates a virtual community by providing a forum-like interface that is moderated by the EB Members but is freely accessible to the scientific community and the general public. It allows all registered users to make comments, raise questions and share experience and expertise on alien species in Europe. Moreover, it provides a means for exchanging opinions and solving disputes in a transparent way. The overall EB activity is commonly agreed upon procedures and standards.
ano.nymous@ccsd.cnrs.fr.invalid (Konstantinos Tsiamis) 28 May 2020
https://hal.inrae.fr/hal-02637388v1
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[hal-03593276] Larvae of longhorned beetles (Coleoptera; Cerambycidae) have evolved a diverse and phylogenetically conserved array of plant cell wall degrading enzymes
Longhorned beetles (Cerambycidae) are the most diverse group of predominantly wood-feeding (xylophagous) insects on Earth. Larvae of most species feed within tissues of plants made up of large amounts of plant cell wall (PCW), which is notoriously difficult to digest. To efficiently access nutrients from their food source, cerambycid larvae have to deconstruct PCW polysaccharides – such as cellulose, hemicelluloses and pectin – requiring them to possess a diversity of plant cell wall degrading enzymes (PCWDEs) in their digestive tract. Genomic data for Cerambycidae are mostly limited to notorious forest pests and are lacking for most of the taxonomic groups. Consequently, our understanding of the distribution and evolution of cerambycid PCWDEs is quite limited. We addressed the numbers, kinds and evolution of cerambycid PCWDEs by surveying larval midgut transcriptomes from 23 species representing six of the eight recognized subfamilies of Cerambycidae and each with very diverse host types (i.e., gymnosperms, angiosperms, xylem, phloem, fresh or dead plant tissues). Using these data, we identified 340 new putative PCWDEs belonging to ten carbohydrate active enzyme families, including two gene families (GH7 and GH53) not previously reported from insects. The remarkably wide range of PCWDEs expressed by Cerambycidae should allow them to break down most PCW polysaccharides. Moreover, the observed distribution of PCWDEs encoded in cerambycid genomes agreed more with phylogenetic relationship of the species studied than with the taxonomic origin or quality of the host plant tissues.
ano.nymous@ccsd.cnrs.fr.invalid (Na Ra Shin) 07 Mar 2022
https://amu.hal.science/hal-03593276v1
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[hal-02659519] Systematics of the Dioryctria abietella species group (Lepidoptera: Pyralidae) based on mitochondrial DNA
Coneworms of the genus Dioryctria Zeller include a number of serious pests of conifer seeds that are notoriously difficult to distinguish as species. We surveyed mitochondrial DNA variation within the abietella species group by sequencing 451 bp of COI and 572 bp of COII genes from 64 individuals of 6 major species in the group. In addition to examining phylogenetic relationships within European members of the group, the study focused on the two most damaging species, D. abietivorella Grote from North America and D. abietella Denis and Schiffermüller from Europe and Asia, which have been considered taxonomically synonymous in the past. In order to detect different levels of divergence, we extensively sampled in seed orchards and natural forests for D. abietella on different hosts. Maximum parsimony and maximum likelihood analyses confirmed the monophyly of the abietella species group and its separation into three clades. The grouping of North American species (Clade A) received strong support in both analyses, whereas relationships between Clade A and the two other European clades were weakly supported. D. simplicella Heinemann could not be unambiguously separated from D. abietella populations. The diverse haplotypes observed in the network analysis conducted with eight populations of polyphagous D. abietella suggested the presence of two distinct lineages in France.
ano.nymous@ccsd.cnrs.fr.invalid (Géraldine Roux-Morabito) 30 May 2020
https://hal.inrae.fr/hal-02659519v1
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[hal-01144571] THE 2014 ALMA LONG BASELINE CAMPAIGN: AN OVERVIEW
A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ~15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried out from September to late November 2014, culminating in end-to-end observations, calibrations, and imaging of selected Science Verification (SV) targets. This paper presents an overview of the campaign and its main results, including an investigation of the short-term coherence properties and systematic phase errors over the long baselines at the ALMA site, a summary of the SV targets and observations, and recommendations for science observing strategies at long baselines. Deep ALMA images of the quasar 3C138 at 97 and 241 GHz are also compared to VLA 43 GHz results, demonstrating an agreement at a level of a few percent. As a result of the extensive program of LBC testing, the highly successful SV imaging at long baselines achieved angular resolutions as fine as 19 mas at ~350 GHz. Observing with ALMA on baselines of up to 15 km is now possible, and opens up new parameter space for submm astronomy.
ano.nymous@ccsd.cnrs.fr.invalid (Alma Partnership) 22 Apr 2015
https://hal.science/hal-01144571v1
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[hal-01577510] Evaluation de risque simplifiée pour les Lethal yellowing type syndromes (LYTS)
La Direction Générale de l’Alimentation du ministère en charge de l’agriculture a saisi l’Anses en vue de la réalisation d’une évaluation du risque simplifiée (ERS) des LYTS. Ce travail a pour but notamment d’évaluer le risque d’introduction et l’impact économique des LYTS dans la zone ARP. La zone ARP à considérer comprend la Martinique, la Guadeloupe, la Guyane, la Réunion et Mayotte.
ano.nymous@ccsd.cnrs.fr.invalid (Michel Dollet) 25 Aug 2017
https://hal.science/hal-01577510v1
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[hal-01577495] Evaluation de risque simplifiée pour Xyllela fastidiosa
En vue de possible échanges sur la présente thématique à un prochain Comité Permanent Phytosanitaire (CPP) à Bruxelles, il est demandé à l’Anses de réaliser une Evaluation du Risque Simplifiée (ERS) pour le 29/06/2012. Cette ERS devra faire notamment un bilan de la situation de Xylella fastidiosa dans le monde, détailler la biologie de la bactérie, son cycle de développement, les conditions météo et exigences climatiques nécessaires à son développement ou l’observation des symptômes, ses plantes hôtes, les moyens de contamination possibles, ainsi que les moyens de contamination possibles, ainsi que les moyens de lutte disponibles.
ano.nymous@ccsd.cnrs.fr.invalid (Charles Manceau) 25 Aug 2017
https://hal.science/hal-01577495v1
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[hal-01578869] Analyse de risque phytosanitaire Ditylenchus dipsaci sur luzerne
Il est demandé à l’Anses de réaliser une ARP sur le nématode des tiges et bulbes (D.dipsaci) sur luzerne (M. sativa).L’ARP consiste à évaluer, selon des normes internationales1, 2 , les preuves biologiques etautres données scientifiques ou économiques pour déterminer si un organisme nuisibledoit être réglementé (ou déréglementé) et la nature des mesures phytosanitaireséventuelles à prendre à son égard. L’ARP s’attache à évaluer l'importance potentielle d'unorganisme nuisible particulier pour une zone clairement définie. Elle peut être initiée pourune ou plusieurs raisons, les plus fréquentes étant : l’identification d'une filière,l’identification d’un organisme nuisible, l’examen ou la révision d'une politique.
ano.nymous@ccsd.cnrs.fr.invalid (Philippe Castagnone-Sereno) 29 Aug 2017
https://hal.science/hal-01578869v1
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[hal-01578870] Méthodes de lutte alternatives à l'épandage aérien de produits phytosanitaires contre les processionnaires du pin et du chêne en conditions urbaines
Contexte : Les chenilles processionnaires sont des insectes défoliateurs que l’on rencontre dans les populations de pins et de chênes et sont les formes larvaires de deux types de lépidoptères : Thaumetopoea pityocampa pour la processionnaire du pin et Thaumetopoea processionea pour la processionnaire du chêne. Leurs pullulations périodiques sont connues dans différentes régions, essentiellement le pourtour méditerranéen et la façade atlantique jusqu’au Pyrénées-Orientales pour la processionnaire du pin, et les régions d’Alsace, de Bourgogne, d’Ile-de-France, du Centre, de Poitou-Charentes et de Midi-Pyrénées pour la processionnaire du chêne. Les chenilles processionnaires de pin et du chêne sont responsables d’une dermite (appelée érucisme) en lien avec les poils urticants et allergisants qui recouvrent leur corps. A l’automne 2011, les professionnels de la forêt privée ont déposé auprès de l’Agence régionale de la santé (ARS) d’Aquitaine des dossiers de demande de traitements par voie aérienne des chenilles processionnaire du pin pour des motifs de protection de la santé publique. Ces demandes concernaient essentiellement des zones urbanisées. L’arrêté du 31 mai 2011 relatif aux conditions d’épandage des produits mentionnés à l’article L. 253-1 de code rural de la pêche maritime par voie aérienne prévoit cependant le respect d’une distance minimale de sécurité de 50 mètres vis-à-vis des habitations et jardins. Les traitements, qui doivent être mis en oeuvre à l’automne, n’ont donc pas été effectués en Aquitaine par voie aérienne en 2011. Dans ces conditions se pose la question des solutions alternatives qui seraient à mettre en oeuvre à l’avenir pour lutter contre les chenilles processionnaires du pin et du chêne dans les zones urbanisées, tant en Aquitaine que dans les autres régions concernées.
ano.nymous@ccsd.cnrs.fr.invalid (Robert Delorme) 29 Aug 2017
https://hal.science/hal-01578870v1
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[hal-04236301] The evolutionary history of capital-breeding moths through the lens of wild silkmoths (Saturniidae) phylogenomics
Wild silkmoths (Saturniidae) are large capital-breeding insects with non-feeding adults that have short lifespan entirely devoted to reproduction. They exhibit a dazzling diversity of sizes, forms, and life-histories inviting questions about the role that key traits may have played in spurring out species diversification and biogeographical movements in organisms with such extreme reproductive strategy. Yet, the absence of a robust phylogenetic framework based on comprehensive taxonomic sampling impedes our understanding of their evolutionary history. We analyzed 1,024 ultraconserved elements (UCEs) and their flanking regions to infer the relationships among 338 species of Saturniidae representing all subfamilies, tribes, and genera. We performed dating and historical biogeographic analyses to reconstruct their evolutionary history in space and time. Rather unexpectedly for a taxonomically well-known family such as Saturniidae, the alignment of taxonomic divisions and ranks with our phylogenetic results led us to propose substantial rearrangements of the family classification. Saturniids most likely originated in the Neotropics, shortly after the Cretaceous-Paleogene extinction event (ca 64.0-52.0 Ma). Old World lineages stemmed from two independent colonization events during the Eocene, presumably through the Bering-Land-Bridge. Extant subfamilies showed limited mobility across biogeographical regions, except for Saturniinae, a subfamily now present on all continents but Antarctica. These results provide a framework for the integration of saturniid evolutionary history into further global studies of biodiversity and conservation, as well as for in-depth investigations of the spatial and temporal dynamics in all lineages and of the role that key innovations played in driving species diversification of these capital-breeding moths.
ano.nymous@ccsd.cnrs.fr.invalid (Rodolphe Rougerie) 10 Oct 2023
https://hal.inrae.fr/hal-04236301v1
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[hal-03419963] Functional and taxonomic responses of tropical moth communities to deforestation
Global insect decline has recently become a cause for major concern, particularly in the tropics where the vast majority of species occurs. Deforestation is suggested as being a major driver of this decline, but how anthropogenic changes in landscape structure affect tropical insect communities has rarely been addressed. We sampled Saturniidae and Sphingidae moths on 27 farms located in Brazilian Amazonia (Para state) and characterised by different deforestation histories. We used functional traits (forewing length, body mass, wing load, trophic niche breadth and resource use strategy), analysed by combining RLQ and null model analyses, to investigate the responses of their taxonomic and functional diversity to landscape change dynamics and current structure. We found that communities had a higher proportion of large and polyphagous species with low wing load in landscapes with low forest quality and relative cover and high land use turnover. This was mainly due to a significant response to deforestation by saturniids, whereas the more mobile sphingids showed no significant landscape-related pattern. We also observed an overall increase of species richness and functional dispersion in landscapes that have been deforested for a long time when compared with more recent agricultural settlements. Our results highlight the complex way in which landscape structure and historical dynamics interact to shape Neotropical moth communities and that saturniid moths respond clearly to the structure of the surrounding landscape, confirming their potential use as an indicator group for environmental monitoring programmes.
ano.nymous@ccsd.cnrs.fr.invalid (Yenny Correa‐carmona) 30 Apr 2022
https://hal.inrae.fr/hal-03419963v1
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[hal-02798025] Modeling the Spatio-temporal Dynamics of the Pine Processionary Moth
This chapter summarizes several modeling studies conducted on the pine processionary moth range expansion in a spatio-temporally heterogeneous environment. These studies provide new approaches for analyzing and modeling range expansions and contribute to a better understanding of the effects of a wide variety of factors on the spatio-temporal dynamics of the pine processionary moth. These dynamics mostly depend on the dispersal, survival and reproduction characteristics of the species, and these characteristics fluctuate in time and space, depending on environmental and biological factors
ano.nymous@ccsd.cnrs.fr.invalid (Lionel Roques) 05 Jun 2020
https://hal.inrae.fr/hal-02798025v1
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[hal-01228847] Analyse de risque phytosanitaire [i]Plasmopara halstedii[/i] agent responsable de la maladie du mildiou du tournesol
absent
ano.nymous@ccsd.cnrs.fr.invalid (Frederic Suffert) 13 Nov 2015
https://hal.science/hal-01228847v1
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[hal-02682063] Approche intégrée de la conservation des populations pyrénéennes de Genévrier thurifère (Juniperus thurifera L.)
Le Genévrier thurifère (Juniperus thurifera L.) est une espèce ligneuse à répartition méditerranéenne, rare dans les Pyrénées où il est distribué en deux populations principales entourées chacune par de petites populations satellites plus ou moins menacées. Ces populations se situent dans des sites proposés au titre de la directive “habitats” pour le réseau Natura 2000. Les thuriféraies françaises sont classées comme “ habitats prioritaires ” par la Directive Habitats de l’Union européenne (code Corine Biotope 42.A27) et l’espèce devrait être inscrite prochainement par l’U.I.C.N. dans la catégorie des espèces vulnérables (V) au niveau mondial. L’objectif du projet est d’approfondir les connaissances sur les populations pyrénéennes afin de mieux évaluer leur valeur patrimoniale et de guider les opérations de gestion in situ, voire de renforcement de population et de conservation ex situ. Il se concrétisera par la rédaction d’un plan de conservation. Pour cela, trois axes d’étude sont mis en oeuvre : l’étude de l’origine et de la dynamique passée des populations pyrénéennes,l’étude de leur dynamique récente et la recherche de facteurs pouvant affecter les capacités de régénération d’une population : rôle de l’avifaune dans la dispersion des graines, influence des ravageurs de galbules sur les potentialités de régénération et influence de champignons mycorhiziens.
ano.nymous@ccsd.cnrs.fr.invalid (Jocelyne Cambecedes) 01 Jun 2020
https://hal.inrae.fr/hal-02682063v1
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[hal-03045949] Grassland-to-crop conversion in agricultural landscapes has lasting impact on the trait diversity of bees
Context Global pollinator decline has motivated much research to understand the underlying mechanisms. Among the multiple pressures threatening pollinators, habitat loss has been suggested as a key-contributing factor. While habitat destruction is often associated with immediate negative impacts, pollinators can also exhibit delayed responses over time. Objectives We used a trait-based approach to investigate how past and current land use at both local and landscape levels impact plant and wild bee communities in grasslands through a functional lens. Methods We measured flower and bee morphological traits that mediate plant–bee trophic linkage in 66 grasslands. Using an extensive database of 20 years of land-use records, we tested the legacy effects of the landscape-level conversion of grassland to crop on flower and bee trait diversity. Results Land-use history was a strong driver of flower and bee trait diversity in grasslands. Particularly, bee trait diversity was lower in landscapes where much of the land was converted from grassland to crop long ago. Bee trait diversity was also strongly driven by plant trait diversity computed with flower traits. However, this relationship was not observed in landscapes with a long history of grassland-to-crop conversion. The effects of land-use history on bee communities were as strong as those of current land use, such as grassland or mass-flowering crop cover in the landscape. Conclusions Habitat loss that occurred long ago in agricultural landscapes alters the relationship between plants and bees over time. The retention of permanent grassland sanctuaries within intensive agricultural landscapes can offset bee decline.
ano.nymous@ccsd.cnrs.fr.invalid (Gaëtane Le Provost) 17 Oct 2023
https://hal.science/hal-03045949v1
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[hal-03686703] Processionnaire du pin et populations endémiques de pins noirs (pin dalmate et pin de Salzmann du Massif central)
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ano.nymous@ccsd.cnrs.fr.invalid (Jérôme Rousselet) 02 Jun 2022
https://hal.inrae.fr/hal-03686703v1
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[hal-02818616] La galerie des génomes séquencés
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ano.nymous@ccsd.cnrs.fr.invalid (Véronique Jorge) 06 Jun 2020
https://hal.inrae.fr/hal-02818616v1
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[hal-02800700] Genèse du projet PORTRAP : Détection précoce et surveillance d'insectes exotiques xylophages dans les sites potentiels d'entrée sur le territoire national.
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ano.nymous@ccsd.cnrs.fr.invalid (Olivier Denux) 05 Jun 2020
https://hal.inrae.fr/hal-02800700v1
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[hal-02625499] Systematics of Phyllocnistis leaf-mining moths (Lepidoptera, Gracillariidae) feeding on dogwood (Cornus spp.) in Northeast Asia, with the description of three new species
During an ongoing DNA-barcoding campaign of the leaf-mining moths that feed on woody plants in Northeast Asia, four lineages of the genus Phyllocnistis (Gracillariidae, Phyllocnistinae) were discovered on dogwood (Cornus spp): P. cornella Ermolaev, 1987 on C. controversa Hemsl. (Japan: Hokkaido) and three new species - one feeding on C. controversa, C. florida L. and C. macrophylla Wall. in Japan (Honshu, Shikoku, Kyushu), a second species on C. macrophylla in China (Yunnan) and a third on Siberian dogwood Cornus alba L. in Russia (Siberia). All these species showed differences in morphology, in the barcode region of the cytochrome c oxidase I gene and in two nuclear genes (histone H3 and 28S ribosomal RNA). No correlation was found between the deep mitochondrial splits observed and the Wolbachia infection pattern. Based on both morphological and molecular evidence, the three recently discovered lineages are described here as new species: P. indistincta Kobayashi & Triberti, sp. n. (Japan), P. saepta Kirichenko, Ohshima & Huang, sp. n. (China) and P. verae Kirichenko, Triberti & Lopez-Vaamonde, sp. n. (Russia). In addition, the authors re-describe the adult morphology of P. cornella, provide the first record of this species from Japan and highlight the diagnostic characters that allow these Cornus-feeding Phyllocnistis species to be distinguished.
ano.nymous@ccsd.cnrs.fr.invalid (Natalia Kirichenko) 26 May 2020
https://hal.inrae.fr/hal-02625499v1
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[hal-01603813] Diversity and Distribution of Leaf Mining Insects on Birches (Betula spp.) in Siberia
The diversity and distribution of leaf mining insects developing on birches (Betula spp.) in Siberia were reviewed based on published records and our observations. Analysis of the literature revealed 52 species of leaf miners recorded as feeding on different Betula species in Siberia. Among them, three species were listed under different names and six species were erroneously recorded as birch consumers. Thus, the revised list of birch leaf miners contains 44 species. Five moth and four sawfly species are mentioned in the literature as pests of Betula. Four sawflies are known to be invasive in North America. Our collections comprised 25 species, including the micro-moth Stigmella continuella (Lepidoptera, Nepticulidae), a new species for Siberia found in Novosibirsk. Immature stages of 15 species were identified using DNA barcoding. Twenty species were recorded from several regions of Siberia for the first time. The dominant group is Lepidoptera (31 species), followed by Coleoptera (7), Hymenoptera (5), and Diptera (1). Two-thirds of all the known leaf miners develop exclusively on birches; the remaining species also colonize alders (Alnus, Betulaceae), some Rosaceae, Salicaceae, and Ulmaceae. In our observations, the majority of insects (96%) developed on B. pendula. About half of them were also observed on the East Asian birches B. dahurica, B. divaricata, B. costata, B. ermanii, and B. gmelinii; five species were found on the North American birches B. occidentalis and B. papyrifera. All the leaf mining species listed in our paper for Siberia also occur in Europe. The similarity between the miner faunas of these regions is discussed and it is warned about possible errors in diagnostics of the Siberian species using the keys and catalogues for the European fauna. The importance of DNA barcoding in the study of the local insect faunas of poorly explored regions is also emphasized.
ano.nymous@ccsd.cnrs.fr.invalid (Natalia Kirichenko) 25 May 2020
https://hal.science/hal-01603813v1
